Optimization of total alkaloids extraction from Actinidia arguta stems using response surface methodology

The total alkaloids from Actinidia arguta stems were extracted and measured using the method of ultrasonic extraction with ethanol as extraction solvent. Response surface methodology was taken to optimize the extraction of total alkaloids in Actinidia arguta stems.Three elements including ethanol concentration, ultrasonic time and solid- liquid ratio were selected to maximize the yield of total alkaloids. Furthermore, the optimal extraction conditions of total alkaloids from Actinidia arguta stems were 72% ethanol, ultrasonic treatment time 30 min, liquidsolid ratio 43.Under these conditions, the content of total alkaloids was 0.542 mg / g from Actinidia arguta stems by ultraviolet spectrophotometer.