Study on separation and purification of total saponins from tartary buckwheat with macroporous resin

In order to optimize process condition of separation and purification of total saponins from tartary buckwheat with macroporous resin. The appropriate SP700 resin was choosed, which the saturated adsorption capacity was up to (25.241±0.590) mg saponins/g resin through the static adsorption and desorption test. The influence of sample liquid concentration, adsorption time on the adsorption capacity, and the effect of ethanol volume fraction on the yield of desorption were studied in the static test. And in the dynamic test, the optimal purification conditions of using SP700 resin to absorb and purify total saponins from tartary buckwheat were as follows:the best concentration of sample solution 0.586mg/m L, velocity 2BV/h, the ratio of resin and sample solution 1 ∶1, in the dynamic elution experiment, segmented elution was carried with 50% and 70% ethanol solution respectively elution velocity 2BV/h, dosage 2³BV, elution rate was up to 88.9%, the content of saponins in eluent was 2 fold as that in sample solution.