Study on the characterization of POD from different cultivars of Chaenomeles spiciosa(Sweet) Nakai

The peroxidase was isolated from Chaenomeles spiciosa( Sweet) Nakai by Tris- HCl buffer.With guaiacol- H2O2 as substrate,the enzyme characterization of three Chaenomeles spiciosa( Sweet) Nakai cultivars was monitored by measuring the absorbance.In YATS 066( A),YATS153( B),YATS251( C),the results showed that the optimum temperature for POD activity were respectively 20,25,30℃.The POD of all cultivars could keep stabilized under 20℃.The peroxidases of three cultivars all had two optimum p H.And the p H stability range was at p H7.5~9,p H3~6,p H3.5~7.Metal ions had the same effects on the POD activity of A,B and C.The optimum inhibitor of POD activity in all three Chaenomeles spiciosa( Sweet) Nakai cultivars was SDS,and then followed by ascorbic acid.