Optimization of cryoprotectants production process for direct vat set Lactobacillus plantarum with high- yield of γ- amino butyric acid

ZHAO Jing; LIANG Jin-zhong

doi:10.13386/j.issn1002-0306.2015.09.022

ZHAO Jing, LIANG Jin-zhong. Optimization of cryoprotectants production process for direct vat set Lactobacillus plantarum with high- yield of γ- amino butyric acid[J]. Science and Technology of Food Industry, 2015, (09): 140-143. DOI: 10.13386/j.issn1002-0306.2015.09.022

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Optimization of cryoprotectants production process for direct vat set Lactobacillus plantarum with high- yield of γ- amino butyric acid

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A cryoprotectant formulation for Lactobacillus plantarum Lp- Lw-131 was optimized using the orthogonal experiment.The optimum cryoprotectant formulation was dried skim milk 15.0g / L,sucrose 20.0g / L,maltodextrin25.0g / L,glycerine 3.0m L / L. The survival rate of Lactobacillus plantarum with the addition of the optimized cryoprotectant formulation after freeze- drying was 75.3%.The electron microcopis observations showed that the protection agent chosen has good protection function on the bacteria.

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Science and Technology of Food Industry

Optimization of cryoprotectants production process for direct vat set Lactobacillus plantarum with high- yield of γ- amino butyric acid

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