Selection of high-yield ferulic acid esterase strain and optimization of enzyme production conditions

Through preliminary and secondary screening,a strains with high enzyme activity which could produce feruloyl esterase was screened out from Fu-brick tea,according to its individual and colony characteristics,it was identified as Eurotium cristatum. Feruloyl esterase enzyme activity was used as measurable indicator,water content,inoculums-size,temperature and time was chosen as variables,the solid-state fermentation conditions of chosen Eurotium cristatum were optimized. The results showed that black primarytea the optimal condition was 1‰ inoculums size,26% moisture content and cultivated at 30℃ for 4 days,the highest enzyme activity reached 142 m U/g in the black Maocha solid-state fermentation experiment.