Enzymolysis technologies and kinetics of Chinese water chestnut(Eleocharis tuberosa) with response surface method

GAO Yi-xia; LU Xing-wang; WU Ya-nan

doi:10.13386/j.issn1002-0306.2015.08.036

GAO Yi-xia, LU Xing-wang, WU Ya-nan. Enzymolysis technologies and kinetics of Chinese water chestnut(Eleocharis tuberosa) with response surface method[J]. Science and Technology of Food Industry, 2015, (08): 215-217. DOI: 10.13386/j.issn1002-0306.2015.08.036

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Enzymolysis technologies and kinetics of Chinese water chestnut(Eleocharis tuberosa) with response surface method

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With dextrose equivalent value(DE) as an index,response surface method was used to investigate the optimum enzymolysis technologies of Chinese water chestnut(Eleocharis tuberosa) starch by alpha-amylase based on single experiment,and its corresponding kinetics parameters were also established. The results indicated that the optimal technologies were time 88 min,temperature 53℃,E/S=0.12U/mg and p H7.7. The verification results of DE reached(53.393%±0.899%) under above conditions. Under p H7.0 and temperature 60℃,Kmand Vmaxwere47.298mg/m L and 0.335mg/(m L·min),respectively,Eaand △H were 11.995 k J/mol and 71.882 k J/mol,respectively.

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Enzymolysis technologies and kinetics of Chinese water chestnut(Eleocharis tuberosa) with response surface method

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