Contents of total plyphenols and total flavones and antioxidant activity of blackberry seed

DPPH,ABTS and ferric ion reducing power methods were used to evaluate in vitro antioxidant activity of blackberry seed. The content of polyphenols was measured using the Folin-Ciocalteau method,and the content of total flavones was measured using the Na NO2- Al Cl3 colorimetric method. Correlation between polyphenol and flavone contents and antioxidant capacity was analyzed. The results showed that ethyl acetate extract and n-butanol extract from blackberry seed had DPPH IC50=( 41.93 ±4.05),( 64.75 ±5.89) μg/m L,respectively and ABTS radical scavenging activityIC50=(2.91±0.46),(3.18 ±1.01)μg/m L,respectively,and ferric ion reducing powerTrolox equivalent=(2348.73±2.78),(1262.55±31.58)μmol/g,respectively. Petroleum ether extract had ABTS radical scavenging activityIC50=(21.85 ±0.61)μg/m L and weak ferric ion reducing powerTrolox equivalent=(123.59±10.01)μmol/g. An excellent correlation between ABTS radical scavenging activity of three extracts and the contents of total polyphenols and total flavones were observed(R2=0.6832 and0.2596). Ferric ion reducing power was correlated with the content of total polyphenols(R2=0.990). Blackberry seed had antioxidant activity.