Effect of colostrum growth factors extracts on proliferation and migration of CaCO-2 cells

In this study,the effect of colostrum growth factors extracts on the proliferation and migration of CaCO-2cells was investigated,which provided a safe and reliable way for the treatment of human gastrointestinal inflammation.Proliferation rate of CaCO-2 cells were determined by MTT assay at 12,24,36 h respectively,CaCO-2total protein content were determinated by BCA reagent. The migration of CaCO-2 cells were determined by scratch assay after given a dose of colostrum growth factors extracts. Results indicated that CaCO-2 cells were related with colostrum growth factors extracts in a way of dose-dependent manner,compared with the control group. Colostrum growth factors with 100 ng/m L could stimulated dramatically the proliferation of CaCO-2 cells at culturing 12 h,by 16.3%( p < 0.05). When the concentration was 1μg/m L,the cells had the maximum proliferation rate by 20.6%. Colostrum growth factors extracts with 1μg/m L could stimulate cells with maximum proliferation at culturing 24,36 h,increased by 30.8% and 40.0%,respectively,but significantly lower than the proliferation rate by the medium containing 10% fetal bovine serum. Cells protein content and migration speed also had related with colostrum growth factors extracts in a way of dose-dependent manner. There were the maximum protein content and migration speed with 10μg/m L.These results demonstrated that colostrum growth factors extracts significantly accelerated cells proliferation rate,increased cell protein content and migration speed.