Optimization of tea polysaccharide extraction from teaseed meal by response surface methodology

Using the degreased teaseed meal as raw material after oil extraction,the extracting process of tea polysaccharide from the teaseed meal was studied,the experiments were carried out to test the yield of tea polysaccharide affected by different factors which including the ratio of liquid to material,concentration of ethanol,extraction time and temperature. Then,the quadratic regression model was established by 4-variable3-level RSM to optimize the four factors. At the same time,ANOVA about the different factors and interaction between them was also tested so as to optimize extraction technology,The optimal conditions to extract tea polysaccharide from the teaseed meal were as follows:the ratio of liquid to material 12 ∶1,the concentration of ethanol 64%,the extraction temperature 50℃ and the extraction time 1.25 h,under these conditions,the extraction yield of tea polysaccharide was 6.43%. The tea polysaccharide yield of the optimization technology was high,and the technology was safe,reliable,the technology provided the theoretical basis for the development and application of tea polysaccharide in food industry.