Study on the cell antioxidant activity of mulberry tea by microwave fixation

Mulberry leaf is a kind of natural plant resources,which is rich in bioactive substance including polyphenols,flavonoid glycoside,polysaccharides and alkaloidals.Fresh mulberry leaves served as raw material to process mulberry tea by microwave fixation.The main quality and antioxidant ingredients including chlorophyll,free amino acids,soluble sugar,polyphenols and flavones of mulberry tea were analyzed. Cell antioxidant activity of different solvent extracts from mulberry tea by microwave fixation was investigated by establishing cell model in this experiment,which called CAA- RBC method for short. The contents of polyphenols and flavones of different solvent extracts of mulberry tea by microwave fixation were 26.53~37.67 mg / g and 18.56~24.89 mg / g,respectively.The ability of cell antioxidant activity of different solvent extracts of mulberry tea was 50% ethanol extract > 75%ethanol extract > 95% ethanol extract > boiling water extract and existed dose effect.Significance analysis showed that the cell antioxidant activity of 50% ~ 75% ethanol extracts were higher than boiling water extract and 95%ethanol extract( p < 0.05).The correlation between cell antioxidant activity and contents of polyphenols and flavones was weak.The coefficient correlation were 0.216,0.129,respectively. So the contents of polyphenols and flavones could not be measured cell antioxidant activity of extracts of mulberry tea.