Study on purification of total flavonoids of corn silk with the Box-behnken design

To optimize the purification of total flavonoids of corn silk,AB-8 macroporous resin was carried out by response surface methodology in this study. Total flavonoids elution rate of corn silk as the indexes,on the basis of single-factor experiments,Box-behnken design was used to explore the optimal conditions. The optimal refined conditions were as follows:55m L sample volume,the velocity of sample solution 1m L/min,2mg/m L sample concentration,adsorption time 2h,4BV of 70% ethanol,elution velocity 1.8m L/min. The recovery efficiency of total flavonoids was up to 80.68%. The purity of total flavonoids increased from 4.15% to 25.68%. Results indicated that the method was simple,convenient,easy to industrial production.