Study on breeding of bacterial cellulose producing strain and fermentation characteristics of mutation strain

DENG Mao-cheng; LI Jing; WANG Yao; CHEN Wei-xin; WU Yong-hui

doi:10.13386/j.issn1002-0306.2015.04.026

DENG Mao-cheng, LI Jing, WANG Yao, CHEN Wei-xin, WU Yong-hui. Study on breeding of bacterial cellulose producing strain and fermentation characteristics of mutation strain[J]. Science and Technology of Food Industry, 2015, (04): 159-162. DOI: 10.13386/j.issn1002-0306.2015.04.026

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Study on breeding of bacterial cellulose producing strain and fermentation characteristics of mutation strain

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In order to obtain high bacterial cellulose(BC)-producing strain,composite mutagenesis of ultraviolet and diethyl sulfate was used to Gluconacetobacter xylinus BC13 which preserved in laboratory. A high-yield mutant strain Y19-11, BC yield reached 15.6g/L, was obtained by mutagenesis, screening and continuous passage. The mutant strain had excellent genetic stability and its BC yield increased by 44.4%,higher than the original strain. Meanwhile,through comparing the cell weight and yield of BC under different temperature and initial pH value,the optimal temperature and initial pH of mutant strain Y19-11 were determined as 30℃ and 5.5,respectively.

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Study on breeding of bacterial cellulose producing strain and fermentation characteristics of mutation strain

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