Simultaneous determination of vitamin A and different configuration of vitamin E in vegetable oils by HPLC

Oils were extracted from white semen sesami nigrum,white peanut,black peanut,sunflower seed,soybean and cotton seed by Soxhlet extraction with mineral ether.Oil samples were dissolved with normal hexane and being a constant volume with methyl alcohol,the content of normal hexane was 20%( v / v) in the solution.The contents of vitamin A,vitamin α- E,vitamin γ-( β) E,vitamin δ- E in the extracted oils were determined on a ODS2 column,using methyl alcohol as mobile phase. Experimental results showed that linear relations of the concentrations of vitamin A,vitamin α- E,vitamin γ-( β) E,vitamin δ- E to peak areas were obtained within0~200μg / m L at 204 nm. The correlation coefficients were more than 0.9978. The average recoveries of vitamin A,vitamin α- E,vitamin γ-( β) E,vitamin δ- E were 97.83%,98.58%,98.75% and 99.93%,respectively.