Study on the optimization of extracting polysaccharides from Eucommia male flowers tea by response surface methodology and the antioxidant evaluation

The optimum extraction process of polysaccharides from Eucommia male flowers tea and its antioxidant activity were explored. The process was designed by Design Expert 8.0,and the extraction conditions were optimized by response surface methodology( RSM).The antioxidant activities were examined from the aspects of scavenging rate of DPPH and hydroxyl free radicals,reducing power,respectively. The results indicated that the optimum extraction conditions were: extraction temperature 90℃,extraction time 4.5h,ratio of solvent to material15∶1. Under these conditions,the yield of polysaccharides had 3.48%.Compared with BHT,the rate of scavenging DPPH radicals was 52.5%,the reducing power reached to 72.73%,and the rate of scavenging hydroxyl radical was63.1% when the Eucommia male flower polysaccharide concentration was 1mg / m L. The above results indicated that the Eucommia male flower polysaccharides had antioxidant effects in vitro.