Optimization of cell preparation technology for high RNA content Beer Yeast QH633

In this paper,we studied the inoculation quantity,the fermentation temperature,the rotation rate and the fermentation time,as the single factor for QH633 fermentation experiment in shaking flask fermentation conditions.Then we selected the optimal parameters,and optimizated the fermentation process through the response surface analysis.The optimal fermentation conditions as follows: fermentation temperature 28℃,inoculation quantity 10.50%( v/v),revolution speed 203r/min,fermentation time 16.5h.Under this condition,the RNA content of QH633 was12.66%,consistented with the predicted value,and compared with 9.38% of the initial fermentation conditions,increased by 34.97%.