Study on microwave- assisted extraction process of polysaccharides and antioxidant activities from Malus halliana koehne

Objective: To select the optimized microwave- assisted extraction process of polysaccharides and inspect its antioxidant activities from Malus halliana koehne.Method: Based on single- factor experiments,Box-Behnken design and its response surface analysis,main factors of affecting polysaccharides yield such as extraction time,power of microwave,the ratio between solvent and material were studied to find the optimal process.Then,the DPPH· scavenging activity,Fe3 +reduction activity,·OH scavenging activity of polysaccharides were studied to inspect its antioxidant activities.Result: The best extraction process was as follows: 30 times of water,extracting 15 min by 3k W power of microwave. When polysaccharides reached the concentration of0.96 mg / m L,the DPPH clearance rat of polysaccharides( extracted by microwave),polysaccharides( extracted by boiling method) was respectively 69.8%,59.5%.The clearance rat of free radical·OH of polysaccharides extracted by microwave and boiling method was respectively 80.1% and 58.2%. Polysaccharides extracted by microwave had better Fe3 +reduction activity.Conclusion: Compared with the traditional methods,microwave extraction method has high efficiency and makes the extracted polysaccharides more bioactive.