Study on separation of anthocyanins from black rice and their antioxidation

Anthocyanins from black rice were separated and their antioxidation was evaluated. Anthocyanin crude extract of black rice was separated to petroleum ether part, ethyl acetate part, n-butyl alcohol part and water soluble part by solvent extraction method. The DPPH radical and hydroxyl radical-scavenging activities were used to determine the antioxidant capacity of each part, the water soluble part was confirmed to have the most significant antioxidation. Then it was separated to five fractions which were C1, C2, C3, C4, C5 by macroporous resin column chromatography method and the DPPH radical and hydroxyl radical-scavenging activities of each fraction were analyzed. The results showed that the antioxidant capacity in a descending order as:C2, C3, C4, C1, C5. Among the water soluble part, the fraction eluted by 20%⁴0% ethanol solution had the highest antioxidant capacity.