Detection for dog-derived components in foods by real-time polymerase chain reaction

GAO Xiao-li; YANG Xin-ting; XUE Chen-yu; WANG Dan; ZHAO Lin-na; HOU Cai-yun

doi:10.13386/j.issn1002-0306.2015.02.004

GAO Xiao-li, YANG Xin-ting, XUE Chen-yu, WANG Dan, ZHAO Lin-na, HOU Cai-yun. Detection for dog-derived components in foods by real-time polymerase chain reaction[J]. Science and Technology of Food Industry, 2015, (02): 61-64. DOI: 10.13386/j.issn1002-0306.2015.02.004

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Detection for dog-derived components in foods by real-time polymerase chain reaction

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A rapid and highly species-specific real-time polymerase chain reaction ( PCR) assay had been developed for the detection of dog-derived components in meat and meat mixtures. Primers and Taqman probe were designed on the mitochondrial ND2, and the performance of the method was tested. Results showed that no cross-reaction was observed between the dog and non-target species, and this method revealed a high sensitivity and could detect 100 fg of dog template DNA. In conclusion, this real-time PCR assay could be a rapid and straightforward method for the accurate identification of dog-derived components in food.

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Science and Technology of Food Industry

Detection for dog-derived components in foods by real-time polymerase chain reaction

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