Establishment of Aminoglycoside resistance gene of foodborne MRSA detection method by Multiple- PCR

Objective: To investigate the prevalence of methicillin- resistant Staphylococcus aureus MRSA and resistance to Aminoglycoside gentamicin,kanamycin in foodborne S.aureus,and establish a rapid quadruple- PCR method for detection and identification antibiotic resistance of S.aureus.Methods: Genomic DNA was extracted as template for PCR from S.aureus recoveried and cultured in enrichment medium.According to nuc for Genus,mec A for MRSA,aac A- aph D and aph( 3')- Ⅲa for Gentamicin and kanamycin,quadruple- RCR detection method was developed,its sensitivity and specificity were evaluated; a total of 72 strains of S.aureus from food and patients with food poisoning were detected by quadruple RCR method. Results: Quadruple- RCR detection method was established.72 strains of S.aureus all had nuc,the coincidence rate of mec A and MRSA was 100%,the coincidence rate of aac A- aph D,aph( 3')- Ⅲa and gentamicin,kanamycin was 95.12%.Conclusion: this established quadruple- RCR method was specific,sensitive. MRSA could be tested rapidly,and there was high accurate rate for detection of gentamicin,kanamycin resistant strains of S.aureus.