Optimization of extraction of phenolic compounds from Gastrodia elata using response surface methodology

Basis on the results of the single factor experiments,Gastrodia elata was used as materials to optimize the extraction conditions of total phenolics by using response surface methodology involved a three leves,three variable Box- Behnken design.The results showed that the optimum polyphenolics extraction conditions were 57%ethanol as extraction solvent,extraction temperature 69℃ and extraction time 53 min.Under the optimum conditions,the experimental polyphenolics content was 44.96 mg GAE/ g of Gastrodia elata tested,which was well matched with the predicted content.Thus,the optimum conditions can be used to extract the polyphenolics from Gastrodia elata.