Purification of xanthohumol by macroporous resin from humulus lupulus and its inhibition activity against DNA oxidative damage

Xanthohumol was separated and purified by macroporous adsorption resin from waste hops (Humulus lupulus L.) , and its inhibition activity against DNA oxidative damage was investigated. The resulted showed that HPD100 A was appropriated to purify xanthohumol from waste hops, with adsorption rate was 100% and desorption rate was 77.69%. The optimal conditions for purification were as follows:the loading concentration of xanthohumol was 0.25mg/m L, the loading volume was 15 BV, the p H of loading solution was 5.5, the loading rate was 2BV/h, the elution was 95% ethanol, the elution rate was 2BV/h and the elution volume was 3BV. Under the optimized conditions, the purity of xanthohumol in extract was increased from 4.95% to 51.50%, with the recover rate was 72.56%. The purified xanthohumol was evaluated its inhibition activity against DNA oxidative damage. The result showed that the purified xanthohumol had dramatically inhibition activity against DNA oxidative damage induced by Phen-Cu SO4-VC, and its inhibition activity (EC50=0.22mg/m L) significantly better than that of ascorbic acid (EC50=0.51mg/m L) and rutin (EC50=0.93mg/m L) .