Separation and purification of D-allose produced by biotransformation

FENG Zai-ping; MU Wan-meng; JIANG Bo; ZHANG Tao; XUE Dong

doi:10.13386/j.issn1002-0306.2014.22.058

FENG Zai-ping, MU Wan-meng, JIANG Bo, ZHANG Tao, XUE Dong. Separation and purification of D-allose produced by biotransformation[J]. Science and Technology of Food Industry, 2014, (22): 304-307. DOI: 10.13386/j.issn1002-0306.2014.22.058

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Separation and purification of D-allose produced by biotransformation

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The anticancer rare sugar D-allose was converted from D-psicose by E. coli BL21/ p ET-22b-tl-rpib.The LC-MS/MS results showed that 25% of D-psicose was converted to D-allose in the reaction product mixture. The p I of the recombinant protein was 6.3 determined by isoelectrofocusing and was successfully applied in isoelectric precipitation. The methods of separation and purification of D-allose was established according to DTF-Ca2 +cation exchange chromatography with a flow rate of 1m L/min, column temperature at60℃ and the loading volume of 4m L.

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Separation and purification of D-allose produced by biotransformation

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