Optimization of polyphenol extraction from Sedum Aizoon L.by response surface methodology

Objective:The main research object was Sedum Aizoon L. Besides, an optimization of extraction process for from Sedum aizoon L. was established. Methods:Based on the single factor analysis, a 3-factor, 3-level Box-Behnken experimental design was applied to evaluate the effect of the three factors, including ethanol concentration, ratio of material to liquid and extraction temperature. Results:The optimal extraction conditions were ethanol concentration of 67% (v/v) , extraction temperature 41℃, extraction time 2h, solid-to-liquid ratio 1∶25 (w/v) . Under such extraction conditions, the experimental yield of polyphenols obtained after the first extraction was up to 60.90mg/g, which was close to the model-predicted value. Conclusion :The established regression equation for polyphenols yield had an excellent goodness of fit. Therefore it was credible and could be used for practical prediction.