Optimization of pectin extraction from banana skin by response surface methodology

Objectives:In order to improve the by-product utilization value of banana skin. Methods:The pectin enzyme in banana skin was inactivated with heat treatment. Extraction solvent was 0.05mol/L hydrochloric acid solution and the p H was adjusted to 2.0 with 0.5mol/L Na OH. Then ultrasonic-assisted extraction of pectin from banana skin with ethyl alcohol precipitation method was optimized. Results:The extraction rate of pectin was4.15%, when the extraction substance with a ratio of liquid to solid 5 ∶1 was processed by ultrasonic wave power 340 W at 76.0℃ for 42.0min before precipitating 1.5h using 95% ethanol and centrifuging 3000r/min15 min. Conclusion:This indicated that pectin could be extracted from by-product of banana skin effectively with this method.