Comparative analysis of antioxidant activity of bamboo leaf extracts of ground bamboo and Phyllostachysnigra var.henon

Six bamboo leaves (five ground bamboos and Phyllostachysnigra var) was used as the materials to analysis the contents of total phenols and total flavonoids by using Forint reduction assay reagent and aluminum nitrate-sodium nitrite colorimetry and their antioxidant activity by using DPPH, ABTS, FRAP and Fenton reaction method, respectively. The result showed that six kinds of bamboo leaves contained abundant botanic secondary metabolites. The yield of extract from Shibataea chinensis Nakai was maximum, up to 8.70%±0.02%, higher than that of extract from Phyllostachysnigra var. The yield of extract from S.nanpingensis Q.F.Zheng et K.F.Huang was 8.02%±0.06%. The content of total phenolics, on dry basis of leaves, varied in a range of 4.13% ⁹.06% in the six kinds of bamboo leaves, total flavonoids located in 2.35% ⁴.37%, respectively.Among the six bamboos, there were more contents of total phenolics and total flavonoids in the extract from Shibataea chinensis Nakai was highest. Meanwhile two species (Shibataea chinensis Nakai and S.nanpingensis Q.F.Zheng et K.F.Huang) revealed the stronger scavenging capacity against DPPH· and ABTS+· than those of any other bamboos.The reducing power in each bamboo leaves were significantly changed (p <0.05) . The reducing power of Phyllostachysnigra var.henon was only lower than that of Shibataea chinensis Nakai, whose FRAP was 585.00μg/m L.