Development of a real-time turbidimeter-based LAMP method for detection of allergen soybean in food

According to the endogenous gene of allergen soybean Lectin, six specific LAMP primers were designed. A loop-mediated isothermal amplification (LAMP) method for detecting allergen soybean in food was established. Real-time monitoring of the LAMP reaction was achieved by a turbidimeter. Specificity, sensitivity, stability and repeatability of this method were tested. The results showed that the method could specifically detect the soybean in food, and the detection sensitivity of the method was 0.1% (w/w) . With the soybean sample of 10%, 1%, 0.1%, 0% (w/w) concentration as templates, stability and repeatability testing was conducted, false negative rate was 0%. The results showed that the LAMP method is suitable for specifically detecting allergen soybean in food.