Determination of sugars in five types of ice wine by high performance liquid chromatography ( HPLC) with evaporative light- scattering detector ( ELSD)

A method was established to determine sugars in wine by using HPLC ( High Performance Liquid Chromatography) with ELSD ( evaporative light- scattering detector) .The samples were separated on a NH2 ( 5μm4.6mm × 25cm) chromatographic co Lumn.The flowing phase was C2H3 N ∶ H2 O = 80 ∶ 20 and the velocity was0.8m L / min. The temperature in column and detector was 40℃ and the input volume was 20μL. Experimental result showed that there were almost two types of sugar in six types of ice wine: fructose and glucose.Fructose content was more than glucose levels, and fructose and glucose content in each type of ice wine were difference.The relative standard deviation of this method was 0.7% ².1% ( n = 6) and the recovery was 94.7% ¹03.6%.The method was accurate and simple, and was suitable for rapid detection glucose and fructose in ice wine.