Optimization of enzymatic hydrolysis of Chinese gooseberry pomace by response surface methodology

Chinese gooseberry pomace was used as raw materials in this paper to study the effect of pectinase on the increased ratios of juice yield by response surface methodology. Based on single factor experiments, the quadric regression equation model was established applying Box- Behnken center composite experimental design, and the correlation coefficient R2 of the model was 0.9636. Through variance analysis and solution of the model, the optimal condition for the hydrolysis of Chinese gooseberry pomace was determined as follows: enzyme temperature 48.98℃, p H3.42, enzyme addition 0.26 mg / g, and hydrolysis time 3.32 h. Under these conditions, the actual increased ratio of juice yield from Chinese gooseberry pomace was ( 44.21 ± 1.22) %, which was closed to the theoretical increased ratio of juice yield of 45.73% with a relative error of 2.46%.