Extraction of total phenols from Suaeda glauca Bge seed and optimization of extraction process by RSM

Total phenols from Suaeda glauca Bge Seed growing on beach was extracted using ethanol as solvent by ultrasonic- assisted extraction ( UAE) method, and extraction process was optimized by Box- Behnken Design in response surface methodology ( RSM) based on single factor experiment, which investigated the influence of temperature, ultrasonic time, solid- liquid ratio and volume fraction of ethanol on total phenolic content ( TPC) . The regression model was built up and interactions between factors were analyzed. The results showed that optimal extraction conditions were 80℃, 80 min, 23g / m L of solid- liquid ratio and 80% of ethanol solvent, and regression model was obtained with 996.94μg / g of theory content of total phenols.Under the optimum conditions, the average value of TPC was 986.03μg / g, RSD% = 1.41.This indicated that the regression model was accurate and reliable for investigation on TPC.