Ultrasonic assisted extraction technology and its antioxidant and tyrosinase inhibition activities of the alkaloid from Zanthoxylum bungeanum

In order to optimize the ultrasonic assisted extraction of alkaloid in Zanthoxylum bungeanum, the single factor and response surface design were applied. The antioxidant was evaluated by 2, 2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, and tyrosinase inhibition activity was conducted by the mushroom tyrosinase inhibition. The results showed that the optimum extracting conditions of Zanthoxylum bungeanum alkaloid were as follows:ethanol concentration 79%, solid-liquid ratio 1∶16g/mL, ultrasonic time 11 min, ultrasonic power 233 W. The IC50 value of the DPPH radical scavenging of Zanthoxylum bungeanum alkaloid was0.0599mg/mL, and the IC50 value of tyrosinase inhibition was 1.212mg/mL. These indicated that Zanthoxylum bungeanum alkaloid exhibited some antioxidant and tyrosinase inhibition activities, but the activities were both lower than VC.