Optimization of extraction process for corn bran protein

In this study, the protein was extracted from corn bran and alkali extraction was selected to optimize by response surface method. The effects of extraction pH, extraction time, extraction temperature, solvent/material ratio and on extraction efficiency were explored. Based on the single-factor experiments, the three key factors, including pH, temperature, solvent/material ratio were selected to design the three-factors-three-levels Box-Behnken experiments design. As a result, a mathematical model was established and the optimum protein extraction condition of corn bran were:extraction time 1.5h, extraction temperature 46℃, pH10, material/solvent ratio 1∶11, resulting in an extraction yield of 79.15%.