Homologous expression of ferulic acid esterase in Aspergillus niger

By using of food-grade Aspergillus niger expression system expressed ferulic acid esterase was an efficient path which could increase production of ferulic acid esterase and reduce production cost. Ferulic acid esterase (faeA) coding region in Aspergillus niger was cloned by the method of PCR, constructed Aspergillus niger expression vector pSZHG-faeA and transformed Aspergillus niger in the use of agrobacterium-mediated method. In the end, 4 positive homologous transformants were screened out, which the faeA gene was integrated into the glucoamylase gene locus. Engineering strain showed that glucoamylase fermentation condition of the engineering strain culture supernatant activity up to 18.6mU/mL. SDS-PAGE analysis showed that the 4 recombination strains were obviously different from starting strain, had about 36 ku interest protein band. The protein expression quality was 188 ²62μg/mL. The result indicated that this reserch realized homologous expression of ferulic acid esterase in Aspergillus niger. This research laid the foundation for mass industrial production of food-grade ferulic acid esterase.