Study on isolation, structural characterization and antioxidant activity evaluation of polysaccharide PP60-S1 from Phellinus pini

The homogeneous polysaccharide PP60-S1 was obtained by water extraction, ethanol precipitation and purified by DEAE-Sepharose F. F. and Sephacryl S-300 from the fruiting bodies of Phellinus pini. The polysaccharide PP60-S1 was composed of glucose, mannose and galactose with a molar ratio of 9.24∶1.00∶0.76, with an average molecular weight of 3.56×104u. The results of methylation analysis indicated that six kinds of glycosidic linkages were detected in the structure of PP60-S1:1-Glc、1, 3-Glc、1, 6-Glc、1, 3-Gal、1, 6-Gal、1, 3, 6-Man, respectively. The main chain contained mainly 1, 6-Glc. Otherwise, the polysaccharide PP60-S1 exhibited a certain degree of antioxidant activity, evaluated by in vitro assay of DPPH·, ABTS+· and ferricreducing antioxidant power (FRAP) .