Study on the preparation of alkali-soluble protein using waste of silver carp after surimi processing

The alkali-soluble protein was prepared by pH-shifting method using waste of silver carp after surimi processing as raw material. The effect of storage of raw material, extracting pH, time, and temperature, ratio of material and buffer, and pH for precipitate on preparation of alkali-soluble protein was also investigated.Results showed that the optimal parameters for preparation were extracting at pH12.0, for 15 min, at 5℃, using two-stage extraction, at ratio of 1∶6 (3+3) , at pH5.5 for precipitate. The recovery of alkali-soluble protein was about 82% under this condition. Different treatment had little difference on the yield of alkali-soluble protein.