Optimization of the extraction technology of Hyodeoxycholic Acid by response surface methodology

Objective:The crude products of Hyodeoxycholic Acid was taken as the raw material, to optimize the extraction technology of Hyodeoxycholic Acid by response surface methodology. Methods:The parameters of extraction technology of Hyodeoxycholic Acid were optimized, with the additive amount of ethyl acetate, the reflow time and reflow temperature as the response factors and yeild rate of Hyodeoxycholic Acid as response value. Results:The effects of factors in order were as follows:the additive amount of ethyl acetate, the reflow time and reflow temperature. The optimal extraction technology was as follows:the additive amount of ethyl acetate was 10∶1 (v∶v) , the reflow time was 2.5h, and reflow temperature was 85℃. Under these conditions, the maximal yield of total content reached 98.78%. Conclusion:The results were accordant with model predictions.The technology could be used as the extraction process of Hyodeoxycholic Acid.