Preparation of antihypertensive peptides from pig scapula protein by enzyme method

Pig scapula protein was hydrolyzed with protease to prepare antihypertensive peptides. The singlefactor method was used to analyze the effect of the kind of enzyme, time, substrate concentration, enzyme/substrate ratio, hydrolysis pH and temperature on degree of hydrolysis and Angiotensin-converting enzyme (ACE) inhibitory activity of pig scapula protein hydrolysate. Operating parameters such as temperature, hydrolysis time and enzyme/substrate ratio were optimized using response surface analysis based on ACE inhibitory rate. The optimal hydrolysis conditions of pig scapula peptide were enzyme / substrate ratio of6900U/g, hydrolysis temperature of 53℃, hydrolysis time of 4.5h. The theoretical value of ACE inhibitory activity was 66.93%, which was close to the actual value of 65.31%.