The development of high cellulase production mutant from Trichoderma Viride and study on the condition of enzyme production

CHEN Ying-lian; FU Zheng; ZHANG Liang-yu; GUAN Bin; KONG Qing

doi:10.13386/j.issn1002-0306.2014.18.032

CHEN Ying-lian, FU Zheng, ZHANG Liang-yu, GUAN Bin, KONG Qing, . The development of high cellulase production mutant from Trichoderma Viride and study on the condition of enzyme production[J]. Science and Technology of Food Industry, 2014, (18): 189-193. DOI: 10.13386/j.issn1002-0306.2014.18.032

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The development of high cellulase production mutant from Trichoderma Viride and study on the condition of enzyme production

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The cellulase production strain T. viride 8140 was treated with UV and EMS. The mutant strain T.viride8140UEUE4-80 was showed to have a higher cellulase activity than others and the Hc of the mutant reached 2.1. After several passages its CMCase and FDAase were stabilized at 21.05U/mL and 2.4U/mL. The conditions of fermentation were studied and the optimal culture medium was decided. Under the optimal culture medium, the CMCase and FPAase of the mutant separately reached 22.5, 2.52U/mL.

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The development of high cellulase production mutant from Trichoderma Viride and study on the condition of enzyme production

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