Preliminary study on apoptosis of MKN-45 cells induced by extracts from Eremurus chinensis Fedtsch.roots

The impact of extracts from Eremurus chinensis Fedtsch.roots ( ECFE) on MKN-45 cells and mechanisms were discussed.Cell viability was determined by the MTT assay.Morphologic changes was evaluated by AO /EB.Mitochondrial membrane potential of cells was detected via JC-1.Western blot was used to analyse signal proteins in the intrinsic pathway of apoptosis.ECFE reduced the MKN-45 cells viability at higher concentrations ( 20, 30 mg /mL) , and the alteration of cells was changed.Meanwhile, mitochondrial membrane potential of cells was declined when cells were treated with 10~30mg /mL ECFE.As such, the expression of Bcl-2 was reduced while Bax, cytochrome c and caspase-3 were all increased. PARP was cut. ECFE inhibit viability of MKN- 45 cells, and induce apoptosis through mitochondrial pathway.