Optimization of protease hydrolysis conditions on silkworm pupa protein

ZHONG Yu-shen; LUO Jin-feng; HUANG Xian-zhi; DING Xiao-wen

doi:10.13386/j.issn1002-0306.2014.17.042

ZHONG Yu-shen, LUO Jin-feng, HUANG Xian-zhi, DING Xiao-wen. Optimization of protease hydrolysis conditions on silkworm pupa protein[J]. Science and Technology of Food Industry, 2014, (17): 232-235. DOI: 10.13386/j.issn1002-0306.2014.17.042

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Optimization of protease hydrolysis conditions on silkworm pupa protein

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Purpose: Enzyme hydrolysis of silkworm pupa protein was studied in order to provide references to deeply exploit silkworm pupa protein.Methods: Protease was used to hydrolyze silkworm pupa protein and spectrophotometry was used to detect indicators.Results: When hydrolyzed by single protease, dedicated protease for silkworm pupa protein showed the highest hydrolysis degree out of 8 proteases used in the experiment, the best hydrolysis conditions were: the substrate concentration was 7%, 53℃, pH7.2, the additive amount of protease was 3%, and the hydrolysis degree turned out to be 21.44%.

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Science and Technology of Food Industry

Optimization of protease hydrolysis conditions on silkworm pupa protein

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