Optimization of conditions of cottonseed protein hydrolyzed by flavourzyme using response surface methodology

Cottonseed protein was hydrolyzed by Flavourzyme. The influences of the substrate /enzyme ratio, hydrolysis time, pH value and temperature on the degree of hydrolysis of cottonseed protein were investigated.On the basis of single factor tests, the best possible extraction parameters were obtained with the response surface methodology ( RSM) , at a three- variable, three- level experiment Box- Behnken design ( BBD) . The optimum hydrolysis parameters were as follows: when the substrate /enzyme ratio was 6000 U /g, the cottonseed protein was hydrolyzed for 4.14 hours at temperature 50℃ under pH 7.45, and the degree of hydrolysis was reached 59.01% ±0.81%.