Optimization of MAPase producing from Zygosaccharomyces rouxii by response surface method

Using MAPase concentration as the response value, Zygosaccharomyces rouxii as the raw material to study the optimal MAPK conditions.Based on the result of single-factor test, response surface methodology with 3factors and 3 levels was adopted according to box- behnken experiment design principle. Two regression equations for the influence relationship of MAPase concentration and three varietals factors were established.On the basis of regression models, the results with surface were simulated to give optimized result and trends of the main factors on the educing sugar concentration.The optimal MAPK conditions were as follows: the temperature was 27℃, salt Concentration 9.28%, time 80.4 hours. Under this condition the MAPase concentration was3.189 ng /mL.