Separation and purification of chlorogenie acid from Lonicera fulvotomentosa with aqueous two-phase systems containing acetone and Dipotassium Hydrogen Phsophate

Chlorogenie acid was separated and purified from Lonicera fulvotomentosa crude extract by acetoneK2HPO4two-phase aqueous system.Influence of material /liquid ratio, acetone /water ratio and salt /liquid ratio on extraction rate and purity of chlorogenie acid was investigated by orthogonal test. The results showed that extraction yield of chlorogenie acid was 91.10% under acetone- water ratio for 8.5∶1.5 ( V/V) , salt- solvent ratio for100∶1 ( mg /mL) and material-solvent ratio for 25∶1 ( mg /mL) , the purity of chlorogenie acid increases from 20.16%with crude extract 42.13%. This method was easy to recovered solvent, dispose and operate, which fit for purification of chlorogenie acid from Lonicera fulvotomentosa.