Molecular identification and enzymology analysis of a acid protease producing strain

HOU Jin-hui; ZHAO Ming-tao

doi:10.13386/j.issn1002-0306.2014.17.024

HOU Jin-hui, ZHAO Ming-tao. Molecular identification and enzymology analysis of a acid protease producing strain[J]. Science and Technology of Food Industry, 2014, (17): 151-155. DOI: 10.13386/j.issn1002-0306.2014.17.024

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Molecular identification and enzymology analysis of a acid protease producing strain

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In the paper, a bacterial strain PX8 with acid protease activity was screened by transparent hydrolysis ring method, and after 16 S rDNA sequence analysis, the strain was identified as Pectobacterium sp.By orthogonal test study concerning pH, temperature, metal ion of enzymatic reactions and fermentation time, acid protease enzyme activity of the strain was analyzed. The results showed, in the condition with 1.2mg /mL Fe2 +, reaction temperature 40℃, pH 4, reaction time 10 min, the enzyme activity reached its peak, 34.625 U /mL.

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Molecular identification and enzymology analysis of a acid protease producing strain

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