Optimization of pre-enrichment protocol of PCR-based method for detection of Escherichia coli

Objective: To compare the enrichment effect of different culture methods and different medium additives on Escherichia coli. Method: This study was to investigate the enrichment effect of 2 culture methods ( static cultivation and shaking cultivation) and 2 medium additives ( lactose and mannitol) on Escherichia coli, and to evaluate the enrichment effect by PCR.Results: The results indicated that shaking cultivation was better than static cultivation.The effect of LB medium adding mannitol and lactose was beneficial to the growth of Escherichia coli.When the dosage of lactose and mannitol were 1.0% and 0.3g /100 mL, the effect of enrichment was best. The enrichment time was 4h.The detection could get good results by using 1.0% lactose and 0.3g /100 mL mannitol with shaking cultivation at 37℃.Conclusion: The enrichment and PCR detection of Escherichia coli can be accomplished in 6h, which can be used for rapid detection of Escherichia coli in foods.