Purification of recombinant antihypertensive peptides VLPVPR by gel and ion exchange chromatography

LI Yan; SUN Hai-yan; ZHOU Li-zhen; LIU Dong

doi:10.13386/j.issn1002-0306.2014.17.015

LI Yan, SUN Hai-yan, ZHOU Li-zhen, LIU Dong. Purification of recombinant antihypertensive peptides VLPVPR by gel and ion exchange chromatography[J]. Science and Technology of Food Industry, 2014, (17): 111-114. DOI: 10.13386/j.issn1002-0306.2014.17.015

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Purification of recombinant antihypertensive peptides VLPVPR by gel and ion exchange chromatography

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To purify the peptide VLPVPR, gel filtration combined with ion exchange chromatography method was employed.Sephadex G-10 was used to desalt firstly.Compared the purification effective of SP Sepharose Fast Flow and Q Sepharose Fast Flow.SP Sepharose Fast Flow was not suitable for VLPVPR purification while Q Sepharose Fast Flow was suitable for VLPVPR purification.The optimization condition of Q Sepharose Fast Flow were pH9.0, feeding volume 10% BV, CHES concertration 10 mmol /L, elute rate 1.0mL /min.The recovery ratio was 93.8% and the purity ratio was 47.2%.The purification effective of Q Sepharose Fast Flow was improved.

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Science and Technology of Food Industry

Purification of recombinant antihypertensive peptides VLPVPR by gel and ion exchange chromatography

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