Real-time PCR method for quantitative detect chicken blending in sheep

XUE Chen-yu; SONG Li-ping; LU Yong; ZHAO Lin-na; WANG Dan; YANG Hong-lian; GUO Miao; YANG Xin-ting; HU Zhi-kai

doi:10.13386/j.issn1002-0306.2014.17.0056

XUE Chen-yu, SONG Li-ping, LU Yong, ZHAO Lin-na, WANG Dan, YANG Hong-lian, GUO Miao, YANG Xin-ting, HU Zhi-kai. Real-time PCR method for quantitative detect chicken blending in sheep[J]. Science and Technology of Food Industry, 2014, (17): 294-297. DOI: 10.13386/j.issn1002-0306.2014.17.0056

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Real-time PCR method for quantitative detect chicken blending in sheep

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A real-time PCR method was provided for quantitative analysis the chicken blending in pig.The primers which target genes were single copy in genome were designed.A series of genome DNA were used as standard curve in real-time PCR.The mathematical conversion parameters about the mass of meat and DNA were determined.The weight /weight equivalents of four blending samples were analyzed.The results showed that the absolute error of detection with a tolerance of less than 5%.This research can provide technical support for food safety.

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Real-time PCR method for quantitative detect chicken blending in sheep

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