Enzymatic hydrolysis condition optimization of cholesterol-lowering silkworm pupa protein peptides based on response surface methodology

YANG Yu-ying; WANG Wei; ZHANG Yu; ZHANG Yi-fan; WU Ying-long

doi:10.13386/j.issn1002-0306.2014.16.031

YANG Yu-ying, WANG Wei, ZHANG Yu, ZHANG Yi-fan, WU Ying-long. Enzymatic hydrolysis condition optimization of cholesterol-lowering silkworm pupa protein peptides based on response surface methodology[J]. Science and Technology of Food Industry, 2014, (16): 175-179. DOI: 10.13386/j.issn1002-0306.2014.16.031

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Enzymatic hydrolysis condition optimization of cholesterol-lowering silkworm pupa protein peptides based on response surface methodology

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Optimization of the hydrolysis conditions of cholesterol-lowering silkworm pupa protein peptides by response surface methodology was performed. The optimized conditions were as follows :enzyme dosage5.1%, hydrolyzing 5 hours at the enzymolysis temperature 52℃ and pH=7.0 with a ratio of substrate/water (w/v) 3.9%, the HMGR inhibitory rate of cholesterol-lowering silkworm pupa protein peptide reached 45.24%. A response surface optimized model based on the cholesterol-lowering silkworm pupa protein peptide extraction conditions was established. The verified test results showed the model with a 97.31% accurate rate.

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Science and Technology of Food Industry

Enzymatic hydrolysis condition optimization of cholesterol-lowering silkworm pupa protein peptides based on response surface methodology

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