Study on the antioxidant activity of walnut pollen extracts

The antioxidant properties of five different walnut pollen extracts (Xilin 2, Xilin 3, Xifu 2, Xiluo 3 and Xiangling) were assayed. The antioxidant activity of methanolic, ethanolic and water extracts were determined using different methods including reducing power, iron (II) chelation, DPPH radical scavenging, anti-lipid oxidation andthe total phenolic contents evaluation. The results showed that statistically significant relationship between the total phenolic content and the reducing power assay, iron (II) chelation assay. The order was methanolic>ethanolic>water. The ability of DPPH radical was Ethanolic>methanolic>water. The water extracted from walnut staminate pollen showed higher TBA activities. Xilin 2 pollen extracts showed the highest scavenging activity for DPPH radicals and Xilin 3 water extracts showed the highest TBA activities.