PCR detection methods of Salmonella and application in vegetables samples

This study had established a PCR method of detection in order for the rapid detection of Salmonella spp.in vegetables. Four pairs of primers were designed for PCR amplification according to specific genes of Salmonella, and the detection limit of Salmonella was determined by the effects of different concentrations of Salmonella bacterial suspension of PCR product bands and the linear relation between the value of OD600 nmand the colony tablet count.The invA genes were screened as stable Salmonella-specific genes, and the detection limit of the PCR technology for Salmonella was determined 1550 cfu /mL and the detection limit of cilantro for Salmonella was 63 cfu /g.Salmonella were detected in six of 180 vegetables samples from Dalian Development Zone by this method, and the detection rate was 3.33%.The experimental results showed that the detection method established could be used to detecte Salmonella in vegetables.