Determination of biogenic amines in commercial bubble chicken legs by HPLC

The samples in experiment derivatized with dansyl chloride.A high performance liquid chromatographic ( HPLC) method was developed for the simultaneous determination of tryptamine, β-phenylethylamine, putrescine, cadaverine, histamine, tyraminespermi- dine, and spermine in commercial bubble chicken legs. The chromatographic sep- aration was carried out on a C18 column. The gradient elution program with a mixture of ultrapure water and acetonitrile at the flow rate 0.8mL /min was used under UV detector with a wavelength of254 nm.Under this experimental condition, 8 amines had a good resolution in 23 min and well linearity ( R2> 0.998) in detection range.The relative standard deviation ( RSD) of precision was lower than 5% respectively. The average recoveries ranged from 90.41% to 99.66% for all amines.The results showed that, with the merits of high sensitivity, precision and well repeatability, the HPLC method was convenient, rapid and reliable for biogenic amines detection. The contents of biogenic amines were ranging between 72.42 and 409.67 mg /kg.Among them, the detection of tryptamine, cadaverine, histamine, putrescine and spermidine was 100%, the average contents were47.53, 26.20, 35.65, 12.94, 8.15 mg /kg.